Beaumont Hospital in Dublin is the principle teaching hospital associated with the RCSI. Other centres for clinical training of RCSI students are James Connolly Memorial Hospital, Blanchardstown, Dublin; Our Lady of Lourdes Hospital, Drogheda; Cavan/Monaghan General Hospital; and Waterford Regional Hospital.

Other hospitals such as Temple Street, Crumlin, Cappagh, the Rotunda, Coombe Women's Hospital and the National Maternity Hospital, Holles Street, Dublin provide specialist clinical experience.

General hospitals in Kilkenny, Navan, Wexford and elsewhere are also actively affiliated to the College in providing a wide variety of supervised and practical work opportunities for students and graduates.