Iran has rejected US charges that it backs terrorist groups such as al-Qaeda and is developing nuclear weapons.
"These sorts of statements are strange political literature and are not fit to be uttered by people who claim to be politicians", an Iranian foreign ministry spokesman said.
"Repeating these accusations does not validate the unfounded claims".
On Wednesday, White House National Security Adviser Ms Condoleezza Rice said Iran was continuing "to engage in a behaviour that is deeply troubling and antithetical to American interests".
She said the US was alarmed by Iran's alleged weapons of mass destruction and nuclear programmes.
Yesterday, the US Secretary of Defence, Mr Donald Rumsfeld, accused Iran are harbouring senior al-Qaeda members.
But Iranian spokesman Asefi replied: "The Islamic Republic of Iran seriously believes in and pursues the principle of combating terrorism, and Iran's nuclear programme, which is clear and unambiguous, has peaceful goals.
"Selective US policies with a double standard in their approach to fighting terrorism leaves the US government in no position to judge others," he said.