1 What happened that prompted Karen to leave her friends in the Sanctuary nightclub on Dumbarton Road around 1am on Sunday morning ?
2 What happened to her when she left the nightclub with a 21-year-old man? Did she go back to his flat at Dorchester Avenue as he told police when he gave investigators a witness statement ?
3 What prompted police to elevate the 21-year-old man from a witness to a suspect in the case? What information did they receive or evidence did they gather to detain him on Wednesday ?
4 Where and when was Karen Buckley killed? Police have recovered a knife from the Forth and Clyde Canal and a post-mortem has been carried out but police have not said how Karen died ?
5 If Karen was killed early on Sunday morning, what did her killer do with her body between then and Monday morning when a car was seen acting suspiciously near High Craigton Farm?